Optogenetics Strains

JAX#

COMMON NAME

PROMOTER

EFFECTOR

EXPRESSION

STRAIN NOMENCLATURE (Oct 2019)

Cre-Inducible Lines

STRAIN NOMENCLATURE

Ai35D

R26 :: CAG

Arch / GFP

Cre-dependent ; opsin/xFP

B6;129S-Gt(ROSA)26Sor^{tm35.1(CAG-aop3/GFP)Hze}/J

Ai40D

R26 :: CAG

ArchT / EGFP

Cre-dependent ; opsin/xFP

B6.Cg-Gt(ROSA)26Sor^{tm40.1(CAG-aop3/EGFP)Hze}/J

2xChETA-tdT

R26 :: CAG

ChETA/ChR2*H134R and tdTom

Cre-dependent ; opsin/xFP

B6;129-Gt(ROSA)26Sor^{tm1(CAG-COP4*E123T*H134R,-tdTomato)Gfng}/J

Ai32

R26 :: CAG

ChR2*H134R / EYFP

Cre-dependent ; opsin/xFP

B6.Cg-Gt(ROSA)26Sor^{tm32(CAG-COP4*H134R/EYFP)Hze}/J

Ai27D

R26 :: CAG

hChR2*H134R / tdTomato

Cre-dependent ; opsin/xFP

B6.Cg-Gt(ROSA)26Sor^{tm27.1(CAG-COP4*H134R/tdTomato)Hze}/J

R26-CAG-LNL-GtACR1-ts-FRed-Kv2.1

R26 :: CAG

GtACR1 / FusionRed

Cre-dependent ; opsin/xFP [soma]

B6;129S6-Gt(ROSA)26Sor^tm3Ksvo/J

Rosa26 CAG-LSL-ReaChR-mCit

R26 :: CAG

ReaChR / mCitrine

Cre-dependent ; opsin/xFP

B6.Cg-Gt(ROSA)26Sor^tm2.2Ksvo/J

Ai39

R26 :: CAG

eNpHR3.0 / EYFP

Cre-dependent ; opsin/xFP

B6;129S-Gt(ROSA)26Sor^{tm39(CAG-hop/EYFP)Hze}/J

R26-CAG-lsl-CaMPARI2

R26 :: CAG

CaMPARI2

Cre-dependent ; calcium indicator / photoconvertible xFP

B6.Cg-Gt(ROSA)26Sor^{tm1(CAG-Eos*/Calm*)Mrc}/J

PC::G5-tdT

RNA pol II :: CAG

GCaMP5G and tdTomato

Cre-dependent ; calcium indicator / xFP

B6;129S6-Polr2a^{Tn(pb-CAG-GCaMP5g,-tdTomato)Tvrd}/J

Ai95D (C57BL/6J)

R26 :: CAG

GCaMP6f

Cre-dependent ; calcium indicator

B6J.Cg-Gt(ROSA)26Sor^{tm95.1(CAG-GCaMP6f)Hze}/MwarJ

R26-Lck-GCaMP6f flox

R26 :: CAG

GCaMP6f [membrane]

Cre-dependent ; calcium indicator [membrane]

C57BL/6N-Gt(ROSA)26Sor^{tm1(CAG-GCaMP6f)Khakh}/J

LSL-Salsa6f

R26 :: CAG

GCaMP6f / tdTom (Salsa6f)

Cre-dependent ;
ratiometric calcium indicator [green/red]

B6(129S4)-Gt(ROSA)26Sor^{tm1.1(CAG-tdTomato/GCaMP6f)Mdcah}/J

Ai96 (C57BL/6J)

R26 :: CAG

GCaMP6s

Cre-dependent ; calcium indicator

B6J.Cg-Gt(ROSA)26Sor^{tm96(CAG-GCaMP6s)Hze}/MwarJ

iMb2-Control-Mosaic::
iChr2-Control-Mosaic

R26 :: CAG

Mb2-mYFP*A206K ; Mb2-tdTomato ; Mb2-mTFP1 ::
H2B-mCherry ; H2B-EGFP ;
H2B-mCerulean

Cre-dependent ;
mosiac xFP [membrane or chromatin/nuclear]

STOCK Gt(ROSA)26Sor^{tm1(CAG-EYFP*,-mEYFP*,-tdTomato*,-mTFP1*)Ben}/Gt(ROSA)26Sor^{tm2(CAG-EYFP*,-mCherry*,-EGFP*,-mCerulean*)Ben}/J

iMb2-Control-Mosaic

R26 :: CAG

Mb2-mYFP*A206K ; Mb2-tdTomato ; Mb2-mTFP1

Cre-dependent ; mosiac xFP [membrane]

STOCK Gt(ROSA)26Sor^{tm1(CAG-EYFP*,-mEYFP*,-tdTomato*,-mTFP1*)Ben}/J

iChr2-Control-Mosaic

R26 :: CAG

H2B-mCherry ; H2B-EGFP ;
H2B-mCerulean

Cre-dependent ;
mosiac xFP [chromatin/nuclear]

STOCK Gt(ROSA)26Sor^{tm2(CAG-EYFP*,-mCherry*,-EGFP*,-mCerulean*)Ben}/J

Rosa26LSL-INDIA

R26 :: CAG

INDIA (mNeonGreen-aCasp3-mRuby2)

Cre-dependent ; fluorescent apoptosis reporter

B6.Cg-Gt(ROSA)26Sor^{tm1(CAG-mNeonGreen/mRuby2)Mnz}/J

TB (tTA2-BFP)

H11 :: CAG

H2B-mTagBFP + tTA2

Cre-dependent xFP [nuclear] and tTA

B6;129-Igs2^{tm5(CAG-tTA2,-TagBFP)Luo}/J

Rosa26-CAGs-LSL-RIK

R26 :: CAG

mKate2 + rtTA3

Cre-dependent xFP and rtTA

B6.Cg-Gt(ROSA)26Sor^{tm2(CAG-rtTA3,-mKate2)Slowe}/J

CAMPER

R26 :: CAG

TEpacVV (cAMP FRET sensor)

Cre-dependent ; fluorescent cAMP reporter

C57BL/6-Gt(ROSA)26Sor^{tm1(CAG-ECFP*/Rapgef3/Venus*)Kama}/J

CAG-Sun1/sfGFP

R26 :: CAG

SunTag / superfolder GFP

Cre-dependent ; inner nuclear membrane tag

B6.129-Gt(ROSA)26Sor^{tm5(CAG-Sun1/sfGFP)Nat}/MmbeJ

Rosa26-LSL-dCas9/SunTag

R26 :: CAG

SunTag(BFP) / dCas9

Cre-dependent ; dCas9 and xFP

STOCK Gt(ROSA)26Sor^{tm1(CAG-cas9*,-BFP)Khk}/Mmjax

SPH

CAG

SunTag-p65-HSF1

Cre-dependent ; dCas9 and xFP

B6;D2-Gm33925^{Tn(pb-CAG-cas9*,-EGFP)1Yangh}/J

Ai34D

R26 :: CAG

Synaptophysin / tdTomato

Cre-dependent ; xFP [synapse]

B6;129S-Gt(ROSA)26Sor^{tm34.1(CAG-Syp/tdTomato)Hze}/J

Ai14

R26 :: CAG

tdTomato

Cre-dependent ; xFP

B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J

Cre:Tet-Inducible Lines from Allen Institute

STRAIN NOMENCLATURE

Ai93D;CaMK2a-tTA

TIGRE :: TRE ; CaMK2a

GCaMP6f + tTA

forebrain calcium indicator before dox

STOCK Igs7^{tm93.1(tetO-GCaMP6f)Hze} Tg(Camk2a-tTA)1Mmay/J

Ai162D

TIGRE :: TRE2 + CAG

GCaMP6s + tTA2s

Cre- and Tet-dependent ; calcium indicator

B6.Cg-Igs7^{tm162.1(tetO-GCaMP6s,CAG-tTA2)Hze}/J

Ai148D

TIGRE :: TRE2 + CAG

GCaMP6f + tTA2

Cre- and Tet-dependent ; calcium indicator

B6.Cg-Igs7^{tm148.1(tetO-GCaMP6f,CAG-tTA2)Hze}/J

Ai94D;CaMK2a-tTA

TIGRE :: TRE ; CaMK2a

GCaMP6s + tTA

forebrain calcium indicator before dox

B6.Cg-Igs7^{tm94.1(tetO-GCaMP6s)Hze} Tg(Camk2a-tTA)1Mmay/J

Ai143D

TIGRE :: TRE

RCaMP1.07

Cre- and Tet-dependent ; calcium indicator [red]

B6.Cg-Igs7^{tm143.1(tetO-RCaMP1.07)Hze}/J

Ai139D

TIGRE :: TRE2 + CAG

EGFP + tdT + tTA2

Cre- and Tet-dependent ; differential xFP

B6.Cg-Igs7^{tm139.1(tetO-EGFP,CAG-tdTomato,-tTA2)Hze}/J

Ai140D

TIGRE :: TRE2 + CAG

EGFP + tTA2

Cre- and Tet-dependent ; xFP

B6.Cg-Igs7^{tm140.1(tetO-EGFP,CAG-tTA2)Hze}/J

Cre:FLP Dual Inducible Lines

STRAIN NOMENCLATURE

Ai80D

R26 :: CAG

CatCh (ChR2*L132C) / EYFP

Cre- and FLP-dependent ; opsin/xFP

B6.Cg-Gt(ROSA)26Sor^{tm80.1(CAG-COP4*L132C/EYFP)Hze}/J

Rosa26 CAG-FSF-LSL-ReaChR-mCit

R26 :: CAG

ReaChR / mCitrine

Cre- and FLP-dependent ; opsin/xFP

B6;129S-Gt(ROSA)26Sor^tm2.1Ksvo/J

RC::FPSit

R26 :: CAG

Synaptophysin / EGFP + tdTomato

Cre- and FLP-dependent ; xFP [synapse]

B6;129S6-Gt(ROSA)26Sort^{m10(CAG-Syp/EGFP*,-tdTomato)Dym}/J

Ai65D

R26 :: CAG

tdTomato

Cre- and FLP-dependent ; xFP

B6;129S-Gt(ROSA)26Sor^{tm65.1(CAG-tdTomato)Hze}/J

RC::FLTG

R26 :: CAG

tdTomato ; EGFP

FLP-dependent tdT ; then Cre-dependent EGFP

B6.Cg-Gt(ROSA)26Sor^{tm1.3(CAG-tdTomato,-EGFP)Pjen}/J

Voltage Indicators : OptoPatch, CaViAr and ASAP2s

STRAIN NOMENCLATURE

Floxopatch, Optopatch2

R26 :: CAG

CheRiff-eGFP :: QuasAr2-mOrange2

Cre-dependent ; all-optical electrophysiology

B6;129S6-Gt(ROSA)26Sor^tm1Acoh/J

Optopatch3 Ai155

TIGRE :: TRE

CheRiff-eGFP :: QuasAr3-mCitrine

Cre- & Tet-dependent ; all-optical electrophysiology

B6;129S6-Igs7^{tm3(tetO-Optopatch3,CAG-tTA)Acoh}/J

Ai169D

TIGRE :: TRE2 + CAG

ASAP2s + tTA2s

Cre- and Tet-dependent ; voltage indicator

B6.Cg-Igs7^{tm169.1(tetO-GFP*/TPTE2*,CAG-tTA2)Hze}/J

Chemogenetic Lines

STRAIN NOMENCLATURE

R26-LSL-Gq-DREADD

R26 :: CAG

activ. Gq-DREADD (hM3Dq) :: mCitrine

Cre-inducible mCitrine ; then CNO-inducible canonical Gq pathway (burst firing)

B6N;129-Tg(CAG-CHRM3*,-mCitrine)1Ute/J

RC::L-hM3Dq

R26 :: CAG

activ. Gq-DREADD (hM3Dq) / mCherry

Cre-inducible mCherry ; then CNO-inducible canonical Gq pathway (burst firing)

B6.Cg-Gt(ROSA)26Sor^{tm3.3(CAG-EGFP,-CHRM3*/mCherry/Htr2a)Pjen}/J

R26-LSL-Gi-DREADD

R26 :: CAG

inhib. Gi-DREADD (hM4Di) :: mCitrine

Cre-inducible mCitrine ; then CNO-inducible canonical Gi pathway (neuron silencing)

B6N.129-Gt(ROSA)26Sor^{tm1(CAG-CHRM4*,-mCitrine)Ute}/J

RC::FPDi

R26 :: CAG

inhib. Gi-DREADD (hM4Di) :: mCherry

FLP-inducible mCherry, then Cre- & CNO-inducible canonical Gi pathway (neuron silencing)

B6;129S6-Gt(ROSA)26Sor^{tm9(CAG-mCherry,-CHRM4*)Dym}/J

Specific Promoter Lines

STRAIN NOMENCLATURE

Thy1-ChR2-EYFP line 18

thymus cell antigen 1

ChR2 / EYFP

widespread brain ; opsin/xFP

B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J

Thy1-ChR2-EYFP line 9

thymus cell antigen 1

ChR2 / EYFP

widespread brain ; opsin/xFP

B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J

ChAT-ChR2(H134R)-EYFP line 6

choline acetyltransferase

mhChR2 / EYFP

cholinergic neurons ; opsin/xFP

B6.Cg-Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/J

VGAT-ChR2(H134R)-EYFP

vesicular GABA transporter

mhChR2 / EYFP

GABAergic interneurons ; opsin/xFP

B6.Cg-Tg(Slc32a1-COP4*H134R/EYFP)8Gfng/J

M72-IRES-ChR2-YFP

olfactory receptor 160

ChR2*H134R / EYFP

M72+ olfactory sensory neurons ; opsin/xFP

STOCK Olfr160^{tm1.1(COP4*/EYFP)Tboz}/J

TRE-GCaMP6s line G6s2

TRE

GCaMP6s

Tet-inducible ; calcium indicator

B6;DBA-Tg(tetO-GCaMP6s)2Niell/J

Snap25-2A-GCaMP6s-D

synaptosomal-assoc. prot.25

GCaMP6s

widespread brain ; calcium indicator

B6.Cg-Snap25^tm3.1Hze/J

Cdh5-GCaMP8

cadherin 5

GCaMP8

blood/brain barrier (endothelial cells)

B6;SJL-Tg(Cdh5-GCaMP8)B20-6Mik/J

Thy1-jRGECO1a-WPRE, line GP8.20

thymus cell antigen 1

jRGECO1a

brain (denser cortex) ; calcium indicator [red]

STOCK Tg(Thy1-jRGECO1a)GP8.20Dkim/J

Thy1-jRGECO1a-WPRE, line GP8.31

thymus cell antigen 1

jRGECO1a

brain (sparser cortex) ; calcium indicator [red]

STOCK Tg(Thy1-jRGECO1a)GP8.31Dkim/J

Thy1-jRGECO1a-WPRE, line GP8.58

thymus cell antigen 1

jRGECO1a

brain ; calcium indicator [red]

C57BL/6J-Tg(Thy1-jRGECO1a)GP8.58Dkim/J

Thy1-jRGECO1a-WPRE, line GP8.62

thymus cell antigen 1

jRGECO1a

brain ; calcium indicator [red]

STOCK Tg(Thy1-jRGECO1a)GP8.62Dkim/J

PrismPlus

Cx3cr1 ::
Snap25 :: Mobp :: Aldh1L1

EGFP ::
YFP :: Cerulean :: DsRedMax

microglia ::
neurons :: oligodendrocytes :: astrocytes

STOCK Cx3cr1^tm1Litt Tg(Prism)1989Htz/J

mito::mKate2

CAG

mKate2

widespread fluorescence in mitochondria

STOCK Tg(CAG-mKate2)1Poche/J

UBC PA-GFP

human ubiquitin C

PA-GFP

widespread photo-activatable xFP

B6.Cg-Ptprca Tg(UBC-PA-GFP)1Mnz/J

Thy1-GCaMP6 Lines from GENIE Project (Janelia/HHMI)

STRAIN NOMENCLATURE

GP4.3

thymus cell antigen 1

GCaMP6s

high (hippocampus); mod (M1 S1 V1)

C57BL/6J-Tg(Thy1-GCaMP6s)GP4.3Dkim/J

GP4.12

thymus cell antigen 1

GCaMP6s

high in M1, amygdala, S1, hippocampus

C57BL/6J-Tg(Thy1-GCaMP6s)GP4.12Dkim/J

GP5.5

thymus cell antigen 1

GCaMP6f

high (hippocampus); mod (M1 S1 V1 amyg ob)

C57BL/6J-Tg(Thy1-GCaMP6f)GP5.5Dkim/J

GP5.11

thymus cell antigen 1

GCaMP6f

moderate in hippocampus, M1, S1, V1, olf. bulb

C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J

GP5.17

thymus cell antigen 1

GCaMP6f

high in hippocampus, M1, S1, V1, amygdala, olf. bulb

C57BL/6J-Tg(Thy1-GCaMP6f)GP5.17Dkim/J